The environmental vibration caused by subway running can affect human health if it exceeds a certain value. Evaluating the exact vibrational response caused by the subway is of great importance. Therefore, an imported vibration meter is used to measure the vibration of Qingdao Metro Line 3 and Shanghai Metro Line 10, respectively. By processing the data, the time-history curves of vibration acceleration are obtained, and the Fourier transform also obtains the corresponding spectrum curves. The spectrum curves are then transformed into the frequency range of the one-third octave spectrum. Finally, the average value of frequency vibration levels is worked out. The differences between Qingdao Metro Line 3 and Shanghai Metro Line 3 in terms of vibration acceleration time-history curves, vibration acceleration spectra, one-third octave spectra, and frequency vibration rates are also discussed. The findings show that the Qingdao Metro’s vibration response is greater than that of Shanghai Metro; the frequency vibration level of Qingdao Metro has surpassed China’s environmental vibration standard, whereas Shanghai Metro’s frequency vibration level is equal to environmental vibration standard.