Site response is one of the key factors affecting the accurate determination of source parameters and robust estimation of high-precision crustal medium attenuation. In this paper, the site responses of 40 short-period observation stations in the active-source observation network of Qilian Mountains were analyzed by using the H/V spectra ratio method. The results showed that the 40 short-period observation stations in the Qilian Mountains active-source observation network have amplification (attenuation) effect in varying degrees. According to the characteristics of coda site response curves of 40 stations, they can be divided into three types:flat, amplification, and high-frequency attenuation. The site response curves of some stations are flat in the frequency band of 1-20 Hz, and the site response value is 1-2 times; Most stations have obvious amplification (attenuation) effect in different frequency bands, and the site response value is 0.3-9 times. The lithology of station foundation may be the main factor determining the characteristics and size of site response curve, which has an inverse correlation with the medium density of station foundation.