To analyze the influence of control points on the adjustment accuracy of regional mobile gravity network, based on the mobile gravity observation data of Jiangsu area, the influence of control points on the adjustment accuracy of gravity network was analyzed from the perspectives of the number and spatial distribution of control points used in the adjustment calculation, and the monitoring ability of Jiangsu gravity network was further analyzed. The results show that when using a single datum point for adjustment calculation, the datum point should be as close as possible to the geometric center of the network or multiple closed loop nodes. When the control points with uniform spatial distribution are adopted, there is no obvious difference in the point value accuracy between the measuring points around the control point and the measuring points in the middle network. Combined with the spatial resolution of Jiangsu regional gravity network and the adjustment accuracy of observation data, it is considered that Jiangsu gravity network has a good monitoring effect on M≥5 earthquakes. The results of this paper can provide a reference basis for reasonably using datum points for adjustment and improving the accuracy of gravity data processing.