A bidirectional laminated shear continuum modeled soil box was developed, and a finite element analysis model of pipe-soil interaction was established to study the dynamic response of buried pipelines under seismic excitation. The seismic response of buried pipelines under transverse non-uniform seismic excitation was analyzed by numerical simulation, and the simulated results were compared with the test results. Results show that the peak strain of pipelines in the simulated and test results is large in the middle and small at two ends; the peak strain in the middle is at least 1.6 times that at two ends. The peak values of pipeline and soil acceleration increase with the loading level, the multipeak frequency gradually diffuses from the frequency domain of 0-10 Hz to 10-20 Hz, and the pipeline moves freely. The displacement of soil increases with the loading level; the slope of the displacement curve decreases when the loading level increases to 0.4 g, and the nonlinear behavior of soil is observed. The conclusions show the rationality of numerical simulation analysis and the reliability of test results, thus providing a basis for studying the effects of transverse non-uniform excitation on the seismic response of buried pipelines.