Wudu-Kangxian fault is one of the regional faults with left-lateral strike slip and thrusting active in southeastern part of Gansu province. Through satellite image interpretation and field investigation, the geometric segmentation and geomorphological and geological characteristics of the fault are studied. The result shows that the fault zone can be divided into the west（F1 ） and east（F2） segments, named Shangbanqiao-Changba fault and Shenjiayuan-Yaoping fault. Since the late Pleistocene, fault activity has formed rich dislocation landform phenomena, such as the left lateral of streams and ridges, terminal facet, fault cliff, saddle, waterfall and ground fissures. The new active of west segment（F1） was in Holocene, occurred from （1. 730 ±0. 111） ka to （1. 670 ± 0. 141） ka ago. Its strong and obvious Holocene active characteristics are found in Ganquan town, mainly shows left-lateral strike slip accompanied by reverse component. The east segment（F2 ） may be a late Pleistocene active fault, mainly shows reverse component accompanied by the left-lateral strike slip.