Based on the observation data of soil gas radon concentration from nine measurement points from 2016 to 2021, the spatial and temporal distribution characteristics of radon concentration intensity in the mid- eastern segment of the Qilianshan fault zone were analyzed. Combining this analysis with strong historical earthquakes, present seismic activities, and fault zone sliding rate, the seismic hazard zones of the mid- eastern segment of the Qilianshan fault zone were determined from a geochemical perspective. The study results show that the study area has stronger soil gas radon concentration intensity in the eastern segment than in the western segment, and the change characteristics of the time series show a decreasing trend. The spatial distribution characteristics of soil gas radon concentration intensity, historical and present seismic activities, and fracture sliding rate are well coupled. This study provides evidence of deep underground fluid activity in the study area, which is vital in tracing the faults and active segments with seismic risk.