To study the influence of isolation and collapse prevention bearings at the end of a stair plate and location of the stairs on the seismic performance of reinforced concrete frame structures, three groups and six models including isolation collapse prevention supports were built using the ETABS software. The effect of frame structures with isolation and collapse prevention bearings and stairwell location on the vibration, internal force, and failure mechanism were analyzed by modal analysis, response spectrum analysis, and pushover analysis. The calculation results show that the location of the stairwell has little impact on torsion of the frame structures after adding isolation and collapse prevention bearings, and the vibration in the two major axes directions of the frame structure is similar, that is, the internal force of the stair columns is significantly reduced. When the layout of the stairwells is in the side span, the internal force of the stair columns is stronger when the frame is subjected to an earthquake in the direction perpendicular to the ladder. The frame beams have improved the structural seismic performance by dissipating the earthquake energy. The overall stability of the stairs can be guaranteed with the isolation and collapse prevention bearings, and the failure of the stairs is delayed during a major earthquake.