During the Nepal M8.1 earthquake on April 25, 2015, abundant co-seismic responses were recorded by many underground fluid stations in China. The underground fluid data observed in Gansu area had co-seismic responses of different degrees as well. In this paper, the co-seismic variation characteristics and response ability of digital water level and water temperature in Gansu area during the earthquake are analyzed. It is found that the co-seismic responses were recorded by six wells (seven measurement items). The co-seismic responses of water level have consistent variation characteristics. The co-seismic responses of water temperature were recorded by only two wells, and the recovery time after earthquake is relatively long.