The microtremor survey can be used to evaluate geologic interfaces with large differences in their geotechnical properties. Using the microtremor survey method to obtain the S-wave velocity profile, we then compared the stratigraphic strata at the Haokou middle school in Qianjiang City with the borehole results obtained by the earthquake monitoring project at the site. The results show that this method has the advantages of high resolution of the stratigraphic structure and good exploration of strata with different geotechnical properties, which can provide an important basis for understanding the stratigraphic stratification.