Because of the limited extrusion of the Tibet Plateau from the northwest to southeast, the Sichuan-Yunnan block has one of the highest concentrations and frequencies of earthquakes in China. It is very critical to assess the seismic hazard and explore the geodynamics of this region by quantifying the slip rates of active faults as well as the earthquake occurrence. The Menglian fault, one of the active left-lateral strike-slip faults in Sichuan-Yunnan block, stretches from China to Myanmar. There are no records of earthquakes on the Menglian fault; however, many earthquakes occurred in its vicinity, which may be related to the activity of the fault, such as the Myanna M_S7.3 earthquake of 1995. In China, the Menglian fault has a length of 90 km and a trend of NE-ENE. The fault shows clear geomorphologic characteristic with linear fault scarps (mainly), fault valleys, and fault trenches. Additionally, various offset streams, gullies, and terraces have been formed along the fault, and the observed minimum left-lateral dislocation is about 7 m. Using high-precision Li-DAR measurement, four typical horizontal dislocation geomorphologies were finely measured. According to the obtained age of the geomorphic surface, the average left-lateral strike-slip rate of the fault since the late Quaternary was (2.2±0.4) mm/a. The results were equivalent to the sliding rate of other NE-trending left-lateral strike-slip faults in southwestern Yunnan, reflecting the overall coordination of regional tectonic activities. According to the maximum left-lateral dislocation of (9.5±1.8) km, the initial time of the left-lateral strike-slip of the fault was estimated to be about (4.7±1.6) Ma, in the middle and late Miocene.