The study of gas geochemical information before and after the earthquake has high application value to monitor and forecast earthquake. In order to understand the relations between the variation of CO and the May 22, 2021 Maduo Ms7.4 earthquake in Qinghai, on the basis of verifying the reliability of Atmospheric InfraRed Sounder (AIRS) date with ground date of Waliguan atmospheric observation station, extract the date of CO from AIRS before and after the Maduo Ms7.4 earthquake, by the sliding mean method and difference method to discuss the relationship between CO changes and seismic activity. The results showed that the extraction of CO geochemical information using satellite remote sensing date is reliable. From the aspect of time, in the first two months of the Maduo Ms7.4 earthquake, the CO concentration gradually increased and recovered to calm after earthquake. From the aspect of space, the CO concentration with particularly obvious changes in the near-surface layer at the epicenter. In March the CO concentration began increase around the epicenter, converged from discrete distribution to the epicenter and seismogenic fault zone in space. The maximun anomaly is 18.60ppbv in the end of April, the center line of the anomalies were consistent with Jiangcuo fault and the distribution of seismic rupture. Excluding the influence of background and seasonal change, inferred the change of CO concentration is caused by earthquake. The abnormal phenomena of CO mainly attributed to underground gases escape, rock compression and collision, and the atmospheric chemical reactions play a secondary role.