The seismic behavior monitoring of groundwater in China has over 40 years history. Cooperated with other precursor means, it has become one important means of all means for earthquake prediction. But the earthquake-reflecting condition of each well and the evaluation of them is different. People have different views on the revocation of Luzhou well in recent years. This paper selects historical target earthquakes based on the earthquake cycle and magnitude in Sichuan and Yunnnan area. We use the method combining qualitative(monthly average method) and quantitative(23 points smoothing method and subordinate function method) approaches to make a systematic analysis on monthly observation data of Luzhou well groundwater level from 1982 to 2014. We found 6 middle-term anomalistic changes, respectively, corresponding to one M_S6.6 earthquake occurred at Batang in 1989 and five strong earthquakes including Wenchuan M_S8.0 earthquake. Among these 6 middle-term anomalies, 2 anomalies belong in the Wenchuan M_S8.0 earthquake. Also, these anomalies were tested by R value. Compared with other wells in Sichuan and Yunnan Province, facts have proved that the middle-term anomalies of Luzhou well water level are obvious, and are not unique case. In addition, the particularity of anomaly before the Wenchuan earthquake and post the Wenchuan earthquake is discussed. That is, second anomaly, and post the Wenchuan earthquake effect is remarkable and lasting. We use the statistical data and the subordinate function of the water level of Luzhou well to calculate the tentative index for middle-term prediction on the occurrence of earthquake. The index can be used to predict the period when a strong earthquake can most likely occur in Sichuan and Yunnan area. Finally, we come up a conclusion that Luzhou well can be used to perform a study on middle-term anomaly and prediction on strong earthquakes while there need to strengthen the management and study of the well. Also, we suggest waterfall can be used as a observed item since waterfall is relevant to the groundwater.